Sterin C, a New Antioxidant from the Mycelial Culture of the Mushroom Stereum hirsutum
نویسندگان
چکیده
Structurally new antioxidative metabolite was isolated from mycelial culture of mushroom Stereum hirsutum. Culture broth was subjected to Diaion HP-20 column chromatography, and 70% aqueous MeOH eluent was extracted with EtOAc. EtOAc extract was purified through silica gel and Sephadex LH-20 column chromatographies, and reversed phase C18 HPLC. Compound was revealed to be new dihydroxylated derivative of sterin B with molecular formula of C12H16O5 (MW 240) by MS and various NMR spectral data analyses, and designated as sterin C. Sterin C showed superoxide radical-scavenging activity with EC50 value of 0.31 mM.
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